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I need help with AutoQuant Colocalization application.

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asked by (120 points) about AutoQuant X2
edited by

I'm using the AutoQuant Colocalization application. In the Colocalization window, there is a scatter plot with the intensity of image A on the vertical axis, and the intensity of image B on the horizontal axis. There is a wedge with three points, forming an angle, with which I can choose thresholds of what is considered to be "colocalized". Does anyone have any suggestions or guide lines on how to choose these thresholds?

1 Answer

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answered by (193k points)

In order to use the Colocalization module of AutoQuant application, I recommend you check the video tutorial from the official web page. From what it seems, you need to open the Colocalization Analysis window from where you can select the thresholds either by using the three points from the histogram or by using the boxes (X1-Y1, X2-Y2, X3-Y3) next to Color Map and Intensity. Once you select them, you need to press the Add button below Operations. I suggest you contact the developers for further information.

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