I have a problem with calibrating images in ImageJ. I know the average intensities (i.e intensity over the whole sample) of my samples from different measurements, and I want to calibrate my images so that the pixel intensities (Gray Values) corresponds to the known intensity. I tried to calibrate images with Analyze > Calibrate by setting the measured mean pixel intensities on the left column and corresponding known average intensities on right (from two different samples). This gives negative minimum values, and obviously this is not what I want. Is there any ways to calibrate the image from known average intensity?