Calibrating intensities.

Question

I have a problem with calibrating images in ImageJ. I know the average intensities (i.e intensity over the whole sample) of my samples from different measurements, and I want to calibrate my images so that the pixel intensities (Gray Values) corresponds to the known intensity. I tried to calibrate images with Analyze > Calibrate by setting the measured mean pixel intensities on the left column and corresponding known average intensities on right (from two different samples). This gives negative minimum values, and obviously this is not what I want. Is there any ways to calibrate the image from known average intensity?

Answer 1

There is a big user guide that teaches you how to configure and use the application. You can access it using the link below. You can use the information on the page for additional intensity configuration. There are also examples of how the configuration options will affect the results.

User guide: http://rsbweb.nih.gov/ij/docs/guide/146-30.html

Additionally, use the help from the websites listed through Google Search: https://goo.gl/178V9s