I want to assign 5 individuals to 1 of 2 reference populations (I know for a fact that they originate from one of these populations). I upload a datafile with both reference pops and another file with the 5 samples to be assigned. I then choose "Assign/Exclude pop as origin of inds" and "assign individuals", a Bayesian method by Rannala&Mountain, enable the probability computation following Paetkau et al. 2004 (10.000 simulated inds, 0.01 type I error rate), and include all 10 microsat loci that I have data on.
The output that I then get, suggests that the probability of my 5 samples to belong to either pop is 0 - which I know not to be true.
Any suggestion as to what I might have done wrong would be highly appreciated? Ultimately I have 2 populations and I would like to figure out if there are any genotypes in popA that are unlikely to originate from popA (suggesting they could be migrants from popB or from another population).