Yes, according to the official information, the software is compatible with the following file systems: Ext4, Ext3, Ext2, FAT32, FAT16, and FAT12 file system based LINUX volumes. You can connect your HDD through USB as it will be detected as a mass storage unit (HDD). After it's detected, start the application and use it to recover data from the USB external HDD. For more information, you can always visit the official website of the application.