Once you are in the rooms from the first floor, click the Calculate Heat Loss button below Heat Loss:
Click the Ceilings tab next to Walls and from the Exposed Ceilings drop-down list click an Insulated Ceiling that meets your requirements:
After that, type the length and the width and enter the thermal resistance for the material that compounds the ceiling. You can find a list with these R values, if you use Google search engine.
Alternatively, while you're working with the rooms from the second floor, select a standard floor covering from the first drop-down menu or custom the materials and layers that compound the floor using the Custom Floor Coverings option:
In this way, the program will automatically use these settings to configure the ceilings from the first floor. I suggest you try this method because you can add the R value for each material, while by the first method you need to calculate an average thermal resistance value manually, by yourself. Contact the support team for further information.