According to the information on the official website, you can use the application with the following file formats: *.adp; *.mdb; *.ade; *.mde; *.mdw; *.accdb; *.accde; *.accdt; *.accdr. There is nowhere mentioned about Access 2016, and I don't think it will be because it hasn't been updated in a very long time.
Note: I have downloaded and run the software on Windows 10 64bit and it works just fine.
Alternatively, you can use MDECompiler because it does the same thing as DB to EXE application. Use the following link to access it: http://mdecompiler.com/