No, the game won't work on Windows 10. By default, it has been developed to run only on the following versions of Windows: Windows 98, Windows Me, Windows 95. If you want a Flight Simulator compatible with Windows 10, then access the link posted below.
There is one version release some time ago (last year) that allows you to play Flight Simulator on newer machines: http://store.steampowered.com/app/314160/
