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Designing primers for Sybr quantification

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asked by about Primer Express
edited by

I use Sybr quantification and therefore don't need any probes. Is there any way to design primers only, without probes? I know I can design both and just take the primers and ignore probes, but making a probe decreases the number of possible primers pairs.

1 Answer

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answered by (194k points)
edited by

There are PDF guides that explain you how to work with the program.

Google Search: https://goo.gl/Pf1axm

Note: Make sure that you have a compatible PDF reader installed on your computer to be able to open the files.

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