Latest questions about "spagedi"

I'm a PhD student, I'm in field "Populations Genetics". I try to use the Spagedi software. However, I do not know how to build the file defining the matrix of genetic distances between alleles.

Hello, may I know what could be the problem if I have the error message below after running the SPAGeDi software? "Specific errors found during the last launch of SPAGeDi.