Yes it will run on Windows 10. I have a client who is running it on three Windows 10 computers. The trick is to go to C:\Program Files\Synemed\EyeScapeNE and RIGHT click on EyeScapeNE.exe and select Properties and click on the Comparability tab and put a check next to Run this program as administrator. Do NOT check anything else. Click Apply and OK. Now EyeScape will run on Windows 10.