I have installed the application and there were no images in the overlay mode. As I've seen in the image viewer, you have to take one image at a time to avoid this "issue". The software will allow you to get the image from the microscope, but you have to save one image at a time. I have checked the HELP database. The procedure is the same. You take one image, then delete it or save it, take another one and the process keeps on going.